Course Code : BBGAA01
This 3 day course would provide hands on and theoretical knowledge to the participant about assembly and characterization of bacterial genome from DNA sequence reads obtained from deep sequencing of bacterial gDNA using Illumina Platform.
Research Scholars / Graduate Students / Faculties who are applying or indenting to use deep sequencing approach for assembly and characterization of bacterial genome using Illumina Platform.
Basic working knowledge on computer (Preference to be given for those exposed to Linux Operating System).
a) To ensure participant to confidently handle deep sequencing data obtained by sequencing bacterial gDNA at beginner level.
b) To give experience to the participant on performing analysis of bacterial gDNA data by assembling / constructing the genome starting from raw data quality control till Assembly. and Annotation of genes in the bacterial genome c) To enable participant to gain command over linux operating system for analysis of deep sequencing data.
a) Introduction to deep sequencing of Bacterial WGS using Illumina Platform
b) Experimental Designs and Quality Control measures for a successful WGS sequencing study
c) Introduction to Illumina based deep sequencing raw data and its quality control
d) Denovo Assembly of HQ Reads to Construct Primary Genome Assembly & Quality Control
e) Scaffolding, Draft Genome Construction & Quality Control
f) Coding & Non-Coding Gene Prediction
g) Annotation & Functional Classification of Predicted Genes
h) Various Possible downstream bioinformatics analysis towards discovery and translational research